FEATURE ARTICLES

The development of new nucleic acid amplification techniques is bringing molecular diagnostics closer to the patient for faster results and improved patient management. A prime example of this near-patient capability is LAMP technology.

Multiple myeloma accounts for about 1% of all types of human cancer and 13% of all haematological malignancies. Much molecular study currently is being undertaken on the disease, as the following selection of articles illustrates.

Louise Jones and Clare Craig reflect on the outputs of a workshop held last year, which looked at how best to handle surgical specimens from the point of removal, such that optimal results will be obtained from molecular analysis as well as conventional microscopy and immunohistochemistry.

The use of BCG is best known as a vaccination against tuberculosis. However, its role in bladder cancer immunotherapy goes back almost four decades, and its success has prompted increasing interest in the manipulation of the immune system against tumours.

Ian Jennings reports on proceedings of the second day of the UK NEQAS for Blood Coagulation scientific and participants’ meeting, which is held annually at The Atrium at Sheffield Hallam University.

Results of recent studies into pre-eclampsia testing have informed the latest National Institute for Health and Care Excellence guidance, which recommends use of the sFlt-1/PlGF ratio test to help rule-out this form of gestational hypertension.

Research in the medical laboratory provides opportunities both for the seasoned professional and also those in the early stages of their career. The work reported below reflects first on an audit of the use of order communications and the need for effective training of clinicians, and second the potential problem of false-positive bloodspot results in inherited metabolic disease screening.

The sixth in this series of articles by Stephen MacDonald on individual aspects of the assessment of uncertainty of measurement considers the impacts created when contributors are not measured in the same units.

Mathew Tomlinson makes a case for SAMi, the latest development in computer-assisted semen analysis, which can provide reliable and reproducible results for sperm concentration and the more objective estimation of motility.

Antimicrobial susceptibility testing methods must be robust, easy to perform, reliable and cost-effective. Here, Becky Walker reports on a comparative study to assess the performance of antibiotic disks and automated zone reading technology.

Recent studies and case reports are driving awareness that biotin can interfere with laboratory testing, producing misleading test results in some documented cases. Patients, laboratories and clinicians may not know about the presence of biotin, so laboratories must decide on appropriate action to reduce potential for diagnostic errors, as Gordon Avery explains.

Different logistical and traceability issues related to tissue block storage problems prompted two laboratories to investigate the benefits of a cost-effective system that offers assurance of quality and security.

Research and the reporting of interesting cases provide publication opportunities both for the seasoned professional and also those in the early stages of their career. The work reported below reflects first on the ongoing research into the role of T follicular helper cell subset analysis in primary immunodeficiency, and second on a patient with undetectable HbA1c which proved to be due to blood loss from a tumour.

The continued increase in the incidence of CPEs is creating workload issues for reference laboratories, but the diagnostics industry is responding with simple, more affordable identification methods that can be adopted by front-line microbiology laboratories, speeding confirmation and reporting.

The importance of treating Aspergillus before it has taken hold, and the challenges currently facing clinical diagnostics laboratories, cannot be over-emphasised. Here, Lewis White and Rosemary Barnes explain their approach to molecular testing and diagnosis.

A PubMed search using the terms ‘Carbapenemase-producing Enterobacteriaceae’ and ‘Disk-diffusion testing’ found many recently published open-access articles, a small selection of which are included below.

What impact could standardised clinical LC-MS/MS assays have on patient care? Here, Professor Brian Keevil talks with Thermo Fisher Scientific market development specialist Sarah Robinson.

Research in the medical laboratory provides opportunities both for the seasoned professional and also those in the early stages of their career. The work reported below focuses first on guidance provided to aid the establishment of defined reference intervals, and second reflects on the implementation and use of large, whole-mount sections and their value in the histopathological assessment of colorectal cancer.

The University Hospital of Wales, like other hospitals around the UK, experienced tissue block storage logistical problems and has now turned to a cost-effective system that offers assurance of quality and security.

Barbara Wild and colleagues from UK NEQAS Haematology examine the responses to a recent abnormal haemoglobin distribution, and also discuss findings from the Liquid Newborn Specimens Scheme.