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Sample inoculum preparation: a UK ring trial of the Inoclic device

Preparation of a correct inoculum is vitally important when performing antibiotic susceptibility testing. The Inoclic sample preparation device is intended to provide a standardised, reproducible inoculum that produces EUCAST-compliant confluent growth. Here, Anne Grayson and Charlotte Duncan present the results of a UK-wide comparative trial.

Disk diffusion has been a much used method for antimicrobial susceptibility testing (AST) in most clinical microbiological laboratories since Bauer et al. first described this technique in the 1960s.1 In the UK this method has been further supported by the British Society for Antimicrobial Chemotherapy (BSAC) guidelines, which were first published in 2001.

However, in 2016, BSAC announced that it would cease active support, maintenance and development of the BSAC method, and instead would focus on helping laboratories in the UK to move over to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) method. There are several differences between each method, including confluent growth as opposed to the semi-confluent growth, different media and antibiotic concentrations.

The EUCAST disk method was developed in 2009. It is a robust and standardised method, providing evidence-based guidance on performing susceptibility

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Online
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IBMS Biomedical Science Congress 2022

International Convention Centre, Birmingham
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Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

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