A look at the CRISPR technology that research scientists use to selectively modify the DNA of living organisms, adapted for use in the laboratory from naturally occurring genome editing systems found in bacteria. Here, Pathology in Practice Science Editor Brian Nation compiles a selection of current research interest.
CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges
Zhou J, Li Z, Seun Olajide J, Wang G. Heliyon. 2024 Feb 14;10(4):e26179. doi: 10.1016/j.heliyon.2024.e26179. eCollection 2024 Feb 29.
CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various CRISPR/Cas systems such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 and CRISPR/Cas3 utilise different mechanisms to detect or differentiate biological activities and nucleotide sequences. Usually, CRISPR/Cas-based nucleic acid detection systems are combined with polymerase chain reaction, loop-mediated isothermal amplification, recombinase polymerase amplification and transcriptional technologies for effective diagnostics.
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