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Microscopy of cutaneous malignancies: an H&E subtype evaluation

Frozen section and H&E staining are an integral part of the support for patients undergoing Mohs micrographic surgery. Here, Jeyrroy Gabriel and colleagues report on a focused assessment of haematoxylin staining sensitivity and specificity.

Non-melanoma keratinocyte carcinomas are on the rise, with around 156,000 cases diagnosed annually in the United Kingdom.1 Basal cell carcinoma (BCC) is the most predominant form encountered.2 In recent years, Mohs micrographic surgery has gained popularity for the treatment of BCC due to the rapid result turnaround, improved surgical results and preservation of healthy tissue.3

The Mohs technique employs mainly haematoxylin and eosin (H&E)-stained frozen sections for surgical margin assessment of cutaneous excisions, utilising microscopic evaluation of the complete, circumferential, peripheral and deep margins.

This study aimed to determine which mordant-based haematoxylin (Ehrlich, Cole, Mayer, Gill I, Gill II, Gill III, Weigert, Harris or Carazzi) produced the optimal morphological clarity of staining for the identification of cellular morphology of cutaneous BCC.

Sample cohort<

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Upcoming Events

analytica 2022

Messe München, Germany
June 21-24, 2022

The Genomic and Microbiology Revolution: In Technology We Trust?

Royal Air Force Museum, Hendon, London
19 July 2022

Arab Lab Live 2022

Dubai International Convention & Exhibition Centre Dubai World Trade Centre - Trade Centre - Trade Centre 2 - Dubai - United Arab Emirates
24 - 26 October 2022

Lab Innovations 2022

Hall 2 NEC, Birmingham
2 & 3 November 2022

MEDICA 2022

Messe Düsseldorf D-40474 Düsseldorf, Stockumer Kirchstraße 61
14 - 17 November 2022

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

Download the FREE Pathology In Practice app from your device's App store

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