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Oligonucleotide-based fluorescence in situ hybridisation

Agilent Technologies has introduced additional Agilent SureFISH probes, creating the largest offering of oligonucleotide-based fluorescence in situ hybridisation (FISH) assays on the market. Over 425 probes are now available, including centromere probes for all 24 chromosomes, and 35 telomere probes.

Agilent SureFISH probes are designed for specific, non-repetitive regions of the genome, providing superior resolution compared to other available technologies. Standard BAC probes are limited in their ability to detect small aberrations, as well as the ability to target chromosomal regions containing repetitive sequences.

Oligo FISH probes enable users to target chromosomal regions quickly, with hybridisation in as little as four hours. An example of the power of oligo technology, Agilent’s SureFISH product line is able to offer centromere probes not typically available in BAC format. This includes centromere probes for chromosomes 5, 13, 14, 19, 21 and 22.

Customers can easily find and purchase SureFISH probes on the SureFISH website, which enables them to search for probes based on chromosome location, cytoband or gene of interest. The chromosome browser also includes images of all probes hybridised at four and 14 hours, allowing users to see the probe performance prior to purchase.

www.agilent.com/genomics/SureFISH

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