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Cell-based gene expression

Applied Biosystems has launched a new family of TaqMan Cells-to-CT kits for cell-based gene expression research. The kits eliminate the timeconsuming step of RNA purification, reducing the amount of time needed to prepare and analyse RNA from cultured cells using real-time polymerase chain reaction (PCR) methods in applications such as siRNA-mediated gene silencing and microRNA analysis.

The TaqMan Cells-to-CT kits allow expression analysis to be performed directly from cultured mammalian cells derived from a variety of tissue sources, including epithelial cells and stem cells. Sample preparation time is reduced to less than 10 minutes, and loss of RNA is eliminated.

The kits incorporate the gold-standard TaqMan chemistry, forming part of a complete workflow that has been designed with all the necessary reagents for expression analysis, and are validated for use with the Applied Biosystems’ TaqMan assays and arrays. The family includes kits for gene expression analysis from mRNA, pre-amplification of selected targets, microRNA expression analysis and gene expression analysis with fast real-time PCR.

www.appliedbiosystems.com

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