Bruker Corporation has launched its new nanoElute 2 nano-LC platform, which supports fluxomics, as well as the latest advances in PaSER’s intelligent acquisition to enhance research in protein-protein interactions (PPIs) and metaproteomics applications.
Launched at the International Mass Spectrometry Conference (IMSC 2022) event, nanoElute includes MetaboScape and TASQ 2023 software.
The next-generation nanoElute 2 combines outstanding nano-LC chromatography performance with hardened high-pressure switching valves, and finger-tight torque-limiting capillaries for leak-free zero dead volume connections, without the risk of overtightening or damaging capillaries. The nanoElute 2 features a further improved nano-LC pump design, and an intuitive and flexible user interface covering a broad range of applications from single-cell proteomics to plasma proteomics. The nanoElute 2 now also supports the label-free ProteoCHIP for the CellenONE platform for high-sensitivity single cell proteomics.
Dr Henrik Johansson, Senior Scientist in Cancer Proteomics in the Department of Oncology-Pathology at the Karolinska Institute in Sweden, said: “The ‘dissolve’ workflow on the nanoElute 2 now perfectly supports our proteomics sample preparation done by the robotic platform in 96- or 384-well plates. The nanoElute 2 dissolves the tryptic peptides directly in the plate prior to injection, avoiding manual intervention and thus streamlining our workflows.”
The latest MetaboScape 2023 release provides streamlined collision cross section (CCS)-enabled metabolic profiling and annotation workflows covering 4D timsTOF data acquisition to highest confidence annotations for lipids and metabolites. New features include improved 4D peak detection and integration, accelerated processing, and smart merging of positive and negative ion data. Powerful automated prediction of CCS values from chemical structures in spectral libraries leverages the accurate CCS values measured on the timsTOF.
In addition, to support the metabolomics community focus on quality, MetaboScape 2023 now offers better means for data quality assessment in large scale studies, while TASQ 2023 extends target screening and quantitation by CCS-enabled MOMA events. Finally, refinements to stable isotope labelling methods simplify large-scale labelling experiments.
Professor Nils Faergeman, Department of Biochemistry and Molecular Biology at the University of Southern Denmark, commented: “The new TASQ fluxomics workflow streamlined our stable isotope labeling experiments on our timsTOF instrument. The integrated natural abundance correction and fractional enrichment calculations together with manual review capabilities enables us to generate results with high confidence for our small molecule isotope tracing studies.”