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New line of antibodies for exosome detection

Biotium, a supplier and inventor of fluorescent cellular probes for life science research, has announced the release of new ExoBrite Antibody Conjugates.

These antibodies were curated specifically for optimal detection of the exosome markers CD9, CD63, and CD81 in isolated exosomes or exosome extracts with little to no background. ExoBrite Antibody Conjugates are available in formulations for both flow cytometry and Western blotting.

Extracellular vesicles (EVs), including exosomes, are increasingly studied for their roles in cell communication, and as potential targets for diagnostic and drug delivery applications. The most common proteins used as exosome markers are CD9, CD63, and CD81, members of the tetraspanin family. While antibodies targeting these proteins are available by commercial suppliers, few are validated or perform well for detection of EVs or exosomes. ExoBrite Flow Antibody Conjugates and ExoBrite Western Antibody Conjugates were developed by Biotium scientists for optimal detection of CD9, CD63, and CD81 exosome markers for flow cytometry and Western blotting.

ExoBrite Flow Antibodies are designed for detection of isolated or bead-bound EVs by flow cytometry. The antibodies are available in four conjugates: ExoBrite 410/450 (Pacific Blue channel), ExoBrite 490/515 (FITC channel), ExoBrite 560/585 (PE channel with yellow laser), and ExoBrite R-PE (PE channel). Isotype controls are also available for each conjugate.

ExoBrite Western Antibodies are designed for detection of exosome markers in EV extracts by near-IR fluorescent Western blot. The antibodies are available with two near-infrared fluorescent dyes, ExoBrite 680/700 and ExoBrite 770/800, which offer greater signal-to-noise for Western blotting than dyes with visible light emission. An ExoBrite Western Antibody for detection of calnexin, a membrane protein localised in the endoplasmic reticulum that is not found in exosomes, is also available as a negative control for assessing purity of exosome extracts.

https://biotium.com/

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Upcoming Events

Pathology Horizons 2024

MacDonald Bath Spa Hotel, Bath
18-20 April, 2024

ECCMID 2024 - European Congress of Clinical Microbiology and Infectious Diseases

Fira Gran Via, 08038 Barcelona, Spain
27-30 April 2024

British Society for Microbial Technology Annual Microbiology Conference

UK Health Security Agency, Colindale, London
2 May 2024

EQA Reports: Interpreting Key Information & Troubleshooting Tips

ONLINE - Zoom
Thursday 16th May 2024

Participants’ Meeting: UK NEQAS Immunology, Immunochemistry & Allergy

Sheffield Hallam University, City Campus, Howard Street, Sheffield
24th May 2024

Med-Tech Innovation Expo

NEC, Birmingham
5-6 June, 2024

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

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