Optimal RNA-Seq library preparation for transcriptome profiling
Bio-Rad Laboratories has announced the launch of the SEQuoia Express Stranded RNA Library Prep Kit, providing an easy-to-use solution for constructing robust libraries to support efficient RNA-Seq workflows for comprehensive transcriptome profiling.
The SEQuoia Express Stranded RNA Library Prep Kit offers a cost-effective and automation-friendly solution to the current challenges in library generation. The three-tube kit uses a novel reverse transcriptase alongside a ligation-free adaptor addition chemistry to yield a reproducible, quantitative RNA-Seq library in three hours. The kit enables researchers to construct robust libraries that capture mRNA and long non-coding RNA (>200 bp) transcripts for differential gene expression analysis and novel transcript discovery. Bio-Rad provides a complementary and customised bioinformatics solution to streamline the full next-generation sequencing (NGS) workflow, from library preparation to data analysis.
“Current RNA library preparation approaches are time-consuming, complex and potentially error-prone, with sample loss observed at each stage in processes with multiple steps,” said Simon May, Executive Vice President and President, Life Science Group, Bio-Rad. “Bio-Rad offers scientists an enhanced solution, combining its rapid NGS library preparation with integrated data analysis to overcome these difficulties and enable the widespread adoption of RNA-based sequencing."