Mounting media with far-red counterstain and fluorescence quenching

Biotium is also releasing EverBrite TrueBlack Hardset Mounting Medium for mounting and quenching of lipofuscin autofluorescence in a single step.

Antifade mounting media, like EverBrite, typically includes DAPI as a convenient blue nuclear counterstain for researchers performing immunofluorescence microscopy. However, cross-talk between fluorescence channels and UV-induced photoconversion of DAPI can cause bleed-through fluorescence in neighboring FITC or Cy3 channels. Biotium developed EverBrite Mounting Media with NucSpot counterstains as a novel solution to DAPI cross-talk and UV photoconversion, offering bright and specific nuclear imaging with very limited interference in other channels. EverBrite NucSpot 640 is specific for the Cy5 channel.

Biotium’s TrueBlack lipofuscin quenchers are unique reagents designed to quench lipofuscin autofluorescence commonly found in human and aged animal tissues. New EverBrite TrueBlack Hardset Mounting Medium is the first reagent to offer both mounting and quenching of lipofuscin autofluorescence in a single step for greater convenience. EverBrite TrueBlack Hardset Mounting Medium will be available with DAPI, NucSpot 640, or without a nuclear counterstain.

This release adds to Biotium’s growing portfolio of novel reagents for immunofluorescence microscopy, offering innovative solutions for all user workflows. Biotium’s new EverBrite with NucSpot and EverBrite TrueBlack Hardset Mounting Medium will be available in a standard 10 mL size or 2 mL trial size. NucSpot 640 nuclear counterstain is currently available with EverBrite Hardset and EverBrite TrueBlack Hardset Mounting Medium. Mounting media variations with NucSpot 680 for the Cy5.5 channel will be released in the coming weeks.

Viisit the EverBrite page on Biotium’s website for the full list of available products.