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New haematoxylin for routine diagnostic use: a comparative study

The idiom ‘to leave no stone unturned’ means to do everything possible to find something or to solve a problem. Here, Guy Orchard and colleagues apply this approach in the search for an alternative mordant for use in a new haematoxylin solution.

Haematoxylin is the most important and widely used dye in cellular pathology. There has not been any significant advance over decades to attempt to improve or develop its use for histological investigations. Traditionally, we have not audited or challenged the existing practices or formulations of the key histological stain we use to evaluate the morphological features of tissue. Accepting practice without challenge or audit is unscientific. No one can expect advancement without reflecting on existing practices to see where improvements can be made.

The aim of this preliminary study is to attempt to see if a new haematoxylin could be made that contains a brand new mordant. One that may impart different staining qualities and offer additional options for histological staining of tissue sections. In this way, future applications can be explored with possible improvements to morphological identification and ultimately pathological diagnosis.

Haematoxylin is a dark blue or violet stain. It is basic and positively charged, and binds to basophilic substances, thus it will bind to DNA and RNA, which are acidic in nature and negatively charged.1–3

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Upcoming Events

POCT Innovators: The Power to Disrupt Series

Cloth Hall Court, Leeds. UK
20 March, 2024

Interpreting Reports: UK NEQAS Haematology

ONLINE
21st March 2024

USCAP 113th Annual Meeting

Baltimore Convention Center, Baltimore, Maryland, USA
23-28 March, 2024

Pathology Horizons 2024

MacDonald Bath Spa Hotel, Bath
18-20 April, 2024

Diagnostics North East Conference 2024

The Catalyst, Newcastle upon Tyne
19 April, 2024

ECCMID 2024 - European Congress of Clinical Microbiology and Infectious Diseases

Fira Gran Via, 08038 Barcelona, Spain
27-30 April 2024

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

Download the FREE Pathology In Practice app from your device's App store

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