Quantification of libraries prepared for Illumina NGS systems

PCR Biosystems has expanded its range of molecular biology kits by introducing the NGSBIO Library Quant Kit for Illumina, which contains everything necessary for quantification of DNA libraries prior to NGS with Illumina platforms.

Employed for the vast majority of next-generation sequencing (NGS), the Illumina system requires quantification of libraries prior to sequencing to ensure the correct amount of library is loaded into the machine. This is essential for generating the maximum amount of clustering data possible per run, saving valuable time and money. An easy-to-see blue version of the kit has also been launched, containing a blue qPCR mix for greater pipetting precision and accuracy.

NGSBIO Library Quant Kit offers consistent and reliable quantification across a wide range of sample types, concentrations, fragment sizes and GC content. The kit contains five DNA standards, primers specific to the P5 and P7 Illumina adapter sequences, a convenient library dilution buffer and qPCRBIO SyGreen Mix or qPCRBIO SyGreen Blue Mix. The blue quantitative polymerase chain reaction (qPCR) mix contains a non-reactive dye to improve reaction mix visibility and is particularly useful when using small reaction volumes or white plates.

PCR Biosystems also provide a free online calculation tool to help scientists quickly and easily analyse results generated with the NGSBIO Library Quant Kit. While anyone can benefit from this tool, it is particularly valuable to those who are new to NGS. Users can download the results in a PDF, or email the results to themselves with the additional benefit of accessing the data online for up to 30 days.

Head of R&D Mark Stevens explained: "As specialists in PCR and other allied technologies, we listen closely to the needs of our customers, and we’ve developed our NGSBIO Library Quant Kit in response. qPCR is the most accurate technique available for library quantification, ensuring optimal cluster densities for improved sequencing efficiency and data quality. NGSBIO Library Quant Kit is designed to not only be highly accurate but also convenient and easy to use."

qPCR offers advantages over other methods as it only measures adapter-bound molecules that can be used as templates for library amplification and cluster generation, as opposed to the quantity of all double-stranded DNA that is present.

https://pcrbio.com/applications/ngs/library-quantification/