Cas9 and dCas9-VPR stable cell lines in CRISPR gene editing workflows
Horizon Discovery Group, a leader in gene editing and gene modulation for cell line engineering, has introduced its stably expressing Cas9 and dCas9-VPR cell lines to help accelerate gene knockout and gene activation experiments, respectively.
The cell lines are optimised to work alongside Horizon’s Edit-R predesigned synthetic single-guide RNA (sgRNA) and CRISPRa guide RNA, offering researchers a complete solution to simplify and streamline CRISPR gene editing and modulation workflows.
Horizon’s Cas9 and dCas9-VPR-stable cell lines were generated using its Edit-R Lentiviral particles with a blasticidin resistance cassette and are provided in pooled format. The cell lines are QC verified and validated to ensure stable expression and functionality of Cas9 or dCas9-VPR endonuclease in a range of common cell backgrounds.
Both cell lines are available in the same background to enable loss- and gain-of-function studies to be performed in parallel, without the need to engineer a cell line specifically for this purpose. Removing the time-intensive step of generating a stable cell line and the cost associated with purchasing a nuclease could help researchers increase R&D productivity and allow novice users gain a better understanding of the CRISPR workflow.
For over 40 years Alpha Laboratories has been providing quality supply and support to UK healthcare professionals for their diagnostic and laboratory product needs. With a continued drive to find new ways to help science improve people’s lives, it focuses on specialist biomarkers such as calprotectin for IBD, alongside routine...