Pipetting solutions improve ELISA workflows

Enzyme-linked immunosorbent assays (ELISAs) are popular plate-based methods to detect and quantify peptides, proteins, antibodies or hormones. However, they generally involve multiple pipetting steps that are time-consuming and tedious to perform, requiring consistent processing to ensure success. INTEGRA Biosciences offers a range of liquid-handling solutions to improve ELISA workflows, designed to make the process easier and results more reproducible.

The VIAFLO 96/384 handheld electronic pipette can improve ELISA workflows by enabling the transfer of samples and reagents into 96 or 384 wells simultaneously. All the steps needed to run the protocol can be saved as a custom program on the instrument, which then guides the user with easy-to-follow prompts. This streamlines the workflow and eliminates user variability. For even higher throughput, it can fill multiple plates quickly using the Repeat Dispense mode.

The ASSIST PLUS pipetting robot can automate the ELISA process – just choose the VIALAB program and steps you need, place the appropriate laboratory ware on the deck, and let the robot do all the work. Pipetting settings and assay set-up options can be modified easily in VIALAB, making sure the system is always perfectly suited to the assay. Various sample and tube types, and multiple reagents, can also be accommodated on the deck, and the choice of pipetting options is huge – you can mount any of INTEGRA’s 25 electronic pipettes onto the ASSIST PLUS.

These solutions are partnered with INTEGRA’s Low Retention GripTips, reducing the residual tip volumes when pipetting buffers that contain surfactants, such as Tween 20. This combination of flexibility and automated, optimised processing helps to guarantee the consistency and reproducibility of ELISAs.  

Visit the INTEGRA Biosciences website to see videos explaining how each platform can assist ELISA workflow.