Fast detection of low-abundance proteins using far-red HI-LED lighting

Syngene has introduced a far-red HI-LED lighting option for its new design of G:BOX Chemi and G:BOX mini multi-application gel and blot imaging systems. This quick-fit, environmentally friendly lighting enables fast workflow and precise detection of IR fluorescently labelled proteins on gels and blots.

With an excitation wavelength of 650-670 nm, the far-red HI-LED light allows G:BOX Chemi and G:BOX mini systems to image sensitive near-IR protein dyes such as IR-Dye 680 and 700, and Alexa Fluor 680 and 700. The new light-emitting diode (LED) light is also more intense than standard LEDs, which means that exposure times are shorter, making the G:BOX system a high-speed imager.

The advantage of adding the new far-red HI-LED light to G:BOX systems, instead of purchasing dedicated IR detection technology, is that users still have the flexibility to image multiple different white and UV light applications. These include 1D/2D DNA and protein gels, as well as chemiluminescent and fluorescent multiplex Western blots, and researchers can generate impressive publication-quality images using just one imaging system.

The eco-friendly far red HI-LED lighting is easy to fit and is controlled automatically via intuitive, icon-driven GeneSys software. This new near-IR lighting combined with the G:BOX system’s real-optical imaging  power, provides excellent resolution and sensitivity comparable to most laser-based systems, allowing accurate detection (femtogram levels) of low-abundance proteins.