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Validation of the QUANTA Flash tTG IgA detection system

Femi Saleh and colleagues assess the value of a new chemiluminescence method to detect antibodies to human tissue transglutaminase in coeliac disease.

Coeliac disease is a small bowel enteropathy, triggered by dietary gluten in genetically susceptible individuals. The detection of immunoglobulin (Ig) A antibodies directed against tissue transglutaminase-2 (tTG2) is a standard first-line investigation for the disease.

            QUANTA Flash IgA tTG is a relatively new method for the detection of tTG IgA antibodies, for use with the BIO-FLASH analyser, a highly automated benchtop instrument that is mostly dedicated to autoimmune and infectious disease serology (Fig 1). The method utilises tTG-coated paramagnetic beads, which are incubated with patient serum before washing and magnetisation; bound antibody is then detected by the addition of isoluminol-conjugated anti-human IgA and trigger reagents, producing a luminescence reaction that is detected by the BIO-FLASH optical system. Relative light emitting units (RLUs) are then converted to chemiluminescence units (CU) with reference to a predefined, lot-specific master curve.

            The objectives of this study are to evaluate the diagnostic performance of QUANTA Flash IgA tTG antibody against the existing method (QUANTA Lite R h-tTG IGA ELISA) and to assess the ability of the system to detect potentially IgA-deficient sera.

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Upcoming Events

Pathology Horizons 2024

MacDonald Bath Spa Hotel, Bath
18-20 April, 2024

Diagnostics North East Conference 2024

The Catalyst, Newcastle upon Tyne
19 April, 2024

ECCMID 2024 - European Congress of Clinical Microbiology and Infectious Diseases

Fira Gran Via, 08038 Barcelona, Spain
27-30 April 2024

British Society for Microbial Technology Annual Microbiology Conference

UK Health Security Agency, Colindale, London
2 May 2024

EQA Reports: Interpreting Key Information & Troubleshooting Tips

ONLINE - Zoom
Thursday 16th May 2024

Participants’ Meeting: UK NEQAS Immunology, Immunochemistry & Allergy

Sheffield Hallam University, City Campus, Howard Street, Sheffield
24th May 2024

Access the latest issue of Pathology In Practice on your mobile device together with an archive of back issues.

Download the FREE Pathology In Practice app from your device's App store

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