Syngene, a leading manufacturer of image analysis solutions, has announced that the lighting and filter conditions in the G:BOX image analysis system have been optimised to allow faster, safer visualisation of proteins on stain-free gels.
Technical specialists at Syngene tested filter and lighting conditions in a G:BOX XR5 image analysis system to image a range of proteins (2–300 ng) run on a Criterion TGX Stain-Free gel. For optimum imaging performance, the gel was exposed to five minutes of mid-wave UV (302 nm) on a UV transilluminator and the gel was imaged using a UV filter and an exposure time of two seconds. The gel was then stained with ProtoBlue Safe and imaged again.
The G:BOX image analysis results from the stained and unstained gel showed that the linearity and sensitivity of both methods of protein visualisation was comparable with proteins being detected in the 2–20 ng range. In fact, with some proteins, the stain-free gels provided better detection limits than the ProtoBlue Safe dye staining. Further details of this research can be found in Application Note 64 on the Syngene website.
www.syngene.com/assets/doc/Technical-notes/Stainfree-gels-64.pdf