BioSilta Oy, a developer of advanced microbial growth systems, has announced the launch of the first products in its EnPresso B range of reagent-based growth systems for bacterial cultures. Extensive results from independent laboratories around the world have confirmed that these new products typically increase protein yields by five- to 100-fold and improve protein functionality when compared to results using conventional growth media.
The EnPresso B range has been developed in response to findings from market research involving over 1200 scientists routinely growing bacterial cultures for the expression of recombinant proteins at laboratory scale. The majority of responders highlighted insufficient yield, followed by quality problems such as poor protein solubility or low activity, as their main challenges.
The products are EnPresso B, EnPresso B Animal-Free (supplied with documentation to certify that the product is free from animal-derived components), and EnPresso B Defined Nitrogen-Free, used to increase the yield of 15N-labelled proteins for nuclear magnetic resonance (NMR) analysis.
For ease of use, the products are supplied as presterilised tablets, which are simply added to sterile, filtered water. Optimised protocols enable researchers to go from preculture to harvest within two days, using standard equipment such as shake flasks or 24-well plates.
www.biosilta.com