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Multiphoton optics for deeper tissue imaging

The new Olympus SCALEVIEW multiphoton microscope objectives, which allow researchers to see deeper into tissue than previously possible, have earned three prestigious life science product awards. The prizes include the 2012 R&D 100 Award and the Gold Edison Award in the Science/Medical category. The SCALEVIEW lenses were also judged to be among the 10 best microscopy innovations of 2012, according to this year’s Microscopy Today Innovation Award competition.

The 25x Olympus 4 mm (NA 1.0) and 8 mm (NA 0.9) SCALEVIEW microscope objectives allow researchers to create highly accurate 3D structural representations of tissue from intact specimens by offering detailed, crisp images over superlong working distances. Using a combination of the cutting edge FluoView FV1000MPE multiphoton microscope system, SCALEVIEW optics and a reagent developed by Dr Atsushi Miyawaki in Japan, which literally makes tissue transparent (Hama H, Kurokawa H, Kawano H et al. Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nat Neurosci 2011; 14 [11]: 1481–8), researchers can see up to 8 mm into the tissue without the need for sectioning.
www.microscopy.olympus.eu

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