The Dittmer Laboratory at the University of North Carolina is using a Freedom EVO 150 with an integrated LightCycler 480 real-time polymerase chain reaction (PCR) system to investigate the biology of viral cancers such as those caused by Kaposi’s sarcoma-associated herpes virus (KSHV). The group uses quantitative PCR (qPCR) profiling to study various aspects of KSHV.
The Freedom EVO system provides walkaway automation of sample preparation for subsequent qualitative and quantitative nucleic acid detection by real-time PCR, ensuring accurate and reliable assay plate set-up in a 384-well format. Post-doctoral research associate Pauline Chugh said: “The workstation gives us the ability to perform automated analysis of up to 750 gene sequences per sample in just one day, allowing us to look, for example, at the whole miRNA library following infection with the virus. Alternatively, we can load 60 individual arrays onto the Freedom EVO and leave the system running overnight.”
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