Olympus has launched a new cellFRAP deck system for the popular IX3 ‘open source concept’ IX83 and IX73 microscopes. Olympus cellFRAP is designed with researchers in mind, providing accurate and flexible live-cell photomanipulation with various evaluation options for data presentation.
Unlike conventional widefield-based fluorescence recovery after photobleaching (FRAP) systems, cellFRAP employs a diffraction-limited laser spot for high-intensity and precise positioning of the bleaching region across the full field of view (FOV). This ensures that photomanipulation reaches every point in the FOV without needing to move the sample. The system’s spot quality allows full flexibility in position and shape of the bleaching region, and enables accurate control of the bleaching area.
Driven by the Olympus real-time controller, the cellFRAP system achieves a short switchover time of only 200 µsec between bleach and post-bleach acquisition, ensuring detection of valuable initial sample response after stimulation.
Incorporated into the Olympus IX3 open source frames, the cellFRAP deck can be adapted to an existing imaging platform with a full range of compatible accessories including the ZDC, incubation chambers, manual or motorised stages and manipulators.
www.olympus-lifescience.com