Malvern Instruments offers an application note that examines the use its NanoSight nanoparticle tracking analysis (NTA) technology in the study of extracellular vesicles (exosomes), where ensuring the stability of sample preparations is essential to more detailed research work. Measuring exosome stability with nanoparticle tracking analysis describes the use of this technology for size and concentration measurements of exosomes stored at 4oC and at room temperature for up to five days.
Apparently ubiquitous in a range of organisms, extracellular vesicles are the subject of intense study and are typically described as exosomes, produced from the cell endosome, or microvesicles, which are the result of cell membrane budding. Current research seeks to provide insight into their role and potential links to clinical conditions. Understanding the stability of samples under study is essential to ensure that any differences are attributable to test conditions and not to sample instability.
The ability of the NanoSight range of instruments to visualise, size and determine the concentration of particles below 300 nm has resulted in it becoming a highly valuable technology in the field of extracellular vesicles.
www.malvern.com/exosome-stability