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Gel imaging of microorganisms in digestive disease

Syngene has announced that its InGenius gel imaging system is being used by scientists at the University of Glasgow to determine the types of microorganism associated with Crohn's disease and coeliac disease.

Researchers in the university's School of Medicine are using a Syngene InGenius gel doc system to visualise microbial DNA stained with SYBR Green on polyacrylamide gels. The information obtained is being used to determine which microflora are associated with the diseases in children, and could help to determine the most effective methods to treat and control the conditions.

Dr Konstantinos Gerasimidis, lecturer in clinical nutrition, commented: "We are looking for microbes in children suffering with digestive diseases and are trying to determine those most prevalent in their colonic samples. We use a variety of molecular microbiology techniques such as electrophoresis gels stained with SYBR Green, which we prefer for visualising DNA, rather than ethidium bromide, because it is significantly more sensitive and less toxic. We decided to upgrade our gel doc to an InGenius system because we have found it to be more sensitive than other imagers."
www.syngene.com

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