Sponsors

Fast probe-based detection of DNA

Thermo Fisher Scientific has launched ABsolute Fast QPCR master mixes for the rapid and reliable probe-based detection of DNA and complementary DNA (cDNA) targets. ABsolute Fast QPCR master mixes have been developed especially for fast quantitative polymerase chain reaction (QPCR) protocols to deliver highperformance QPCR results, on all major QPCR platforms, in a fraction of the time required previously.

By minimising QPCR protocol time, ABsolute Fast QPCR master mixes maximise throughput while delivering reproducibility, sensitivity and specificity. The mixes contain Thermo-Fast DNA polymerase, which can be activated in just five minutes at 95ºC. Further time savings are achieved by optimising the temperature of the combined annealing and extension step, and significantly reducing dwell times at each temperature, allowing the fast QPCR protocol to be completed in less than one hour.

ABsolute Fast QPCR master mixes improve the performance of fast QPCR reactions and demonstrate increased sensitivity, superior reproducibility of replicates and a more linear dynamic range.

Thermo Fisher Scientific
www.thermofisher.com

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