Qiagen has launched a new product that supports the genomic analysis of fixed tissue samples. The REPLI-g FFPE kit, the latest development in QiagenÆs whole-genome amplification portfolio, eliminates problems associated with DNA fragmentation and damage caused by formalin fixation.
The REPLI-g FFPE kit provides uniform amplification, resulting in scalable and standardised DNA yields. Following lysis of the tissue section, the DNA is processed using special buffers and enzymes that ligate fragmented DNA. The long DNA strands created by the ligation reaction are amplified using proven REPLI-g technology. A two-hour amplification reaction typically yields 10 µg DNA, whereas 40 µg can be obtained after eight hours’ incubation. Once amplified, the DNA is suitable for immediate use in most downstream genotyping assays without further purification.
Qiagen www.qiagen.com