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Haemoglobinopathy screening by capillary electrophoresis

The comprehensive test menu on SebiaÆs Capillarys system now extends to the identification and quantification of haemoglobinopathies, including Γ-thalassaemia and sickle cell anaemia.

With the expansion of national antenatal and neonatal screening programmes, accurate measurement of haemoglobin variants is becoming increasingly important in routine haematology.

A recent study carried out at the Central Middlesex Hospital, London, demonstrated excellent correlation between capillary electrophoresis and high-performance liquid chromatography (HPLC) methods for the measurement of multiple haemoglobin fractions.

An advantage of using capillary electrophoresis for this type of analysis is the ability to quantify HbA2 in the presence of HbE and HbS, which is normally not possible with conventional HPLC methodology.

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