Time-saving polymerase chain reaction buffer

The Thermo Scientific ReddyMix PCR buffer is specially formulated with a red tracker dye and precipitant in the reaction buffer. The red dye migrates between bromophenol blue and xylene cyanol at approximately 300 bp, depending on the agarose concentration. As the additives are in the reaction buffer rather than in the enzyme preparation, the red dye and precipitant are present at optimal concentrations in the final mix.

By reducing pipetting and reagent handling steps in the PCR process, use of ReddyMix reduces contamination risks and enhances testing reproducibility. This means that standard tests can be set up for screening or teaching purposes, enabling users to have confidence in the consistency of results.

ReddyMix has no adverse effect on the outcome of PCR: yields are the same as those achieved with standard polymerase products, and use of the product does not interfere with downstream applications. ReddyMix is available across the whole Thermo Scientific PCR reagent range.

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