Comprehensive miRNA profiling by real-time PCR

Leading the way in this exciting field, QIAGEN has added to its miRNA portfolio with the introduction of the miScript system, which detects and quantifies miRNA, mRNA and other small RNAs using the SYBR Green-based realtime polymerase chain reaction (PCR).

The miScript system can quantify multiple miRNAs from a single cDNA synthesis reaction, reducing variability and saving precious samples. Both miRNA and mRNA can be quantified from the same cDNA synthesis reaction, enabling simultaneous detection of reference genes or other mRNAs of interest, such as the mRNA targeted by an miRNA.

The miScript system is highly specific, overcoming the challenges presented by the existence of multiple miRNA isoforms. In addition, high sensitivity provides a dynamic range several orders of magnitude greater than microarrays, ensuring comprehensive and accurate miRNA profiles.

www.qiagen.com/mirna