One of the biggest obstacles to acquiring meaningful live-cell time-lapse data has been swept away with the launch of the new real-time focus facility on the Nikon TE2000-PFS motorised inverted microscope.
Stable in-focus observations over an extended period of time are possible with the TE2000-PFS, as it automatically detects the surface of the coverslip optically and continually corrects the focus to compensate for even the most infinitesimal change. This means that the user no longer has to adjust the focus each time stage movement occurs, or after adding reagents. As focus adjustment is no longer necessary, photobleaching is also minimised; thus, cells can be kept alive for longer during fluorescence imaging. NikonÆs PFS is compatible with a wide range of observation methods, from brightfield and fluorescence microscopy to DIC, phase contrast and others.